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Violence: When a tourist is told that his Greek T-shirt announces he's gay (in a crass way), he attacks the tour guide who gave it to him, pushing him into a pool and yelling at him.
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Sex: Several references to Georgia's inactive sex life; a few bawdy jokes about the easily available Spanish divorcees on the tour. Several characters flirt and kiss, and one couple ends up going to bed together (they're shown, torsos covered, on the floor). It's implied that another character has a menage a trois, because his hotel door opens and he's in boxers and a robe and then two women in lingerie are visible behind him.
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Language: Relatively mild for a PG-13 film: "ass/es," "idiot," "oh my God," "hell," "damn," "suckers," "stupid," and the words "his stick" misheard as "his d--k." A character's nickname is Poupi, and his last name is Kakas ("Poupi Kakas"; his nephew is "Doudi Kakas").
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Consumerism: One of the characters is an exec at IHOP (International House of Pancakes), and he mentions IHOP constantly, gives everyone his business cards, and compares people and places to menu items. It's a running joke throughout the film. Another tour guide discusses taking his group to the Hard Rock Cafe.
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Drinking, drugs and smoking: Adults frequently drink beer, cocktails, and Ouzo on their Greek vacation. In one scene, everyone's at a bar, and a few characters get drunk. One tourist pulls out a cigar but is told he can't smoke it.